Fluorescence-Based Assays for Measuring Doxorubicin in Biological Systems

  • Melinda K. Kauffman Washington and Lee University, Lexington, VA 24450, USA
  • Megan E. Kauffman Campbell University Jerry M. Wallace School of Osteopathic Medicine, Buies Creek, NC 27506, USA
  • Hong Zhu Campbell University Jerry M. Wallace School of Osteopathic Medicine, Buies Creek, NC 27506, USA
  • Zhenquan Jia Department of Biology, University of North Carolina, Greensboro, NC 27412, USA
  • Y. Robert Li Campbell University Jerry M. Wallace School of Osteopathic Medicine, Buies Creek, NC 27506, USA; Department of Biology, University of North Carolina, Greensboro, NC 27412, USA; Virginia Tech–Wake Forest University School of Biomedical Engineering and Sciences, Blacksburg, VA 24061, USA; Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Keywords: Fluorescence, Flow cytometry, Doxorubicin, Microscopy, Spectrometry

Abstract

Detection and measurement of doxorubicin in biological systems, including body fluids, cells, and tissues, are instrumental in understanding the mechanisms of action of this widely used drug in treating cancer as well as in causing adverse effects. In this article, we, for the first time, characterized the use of fluorescence-based techniques, including fluorescence spectrometry, microscopy, and flow cytometry in measuring and/or detecting doxorubicin in biological systems, including cell lysates and cultured intact cells. We showed that doxorubicin has a maximum excitation and emission wavelength of 470 and 560 nm, respectively. The detection sensitivity by fluorescence spectrometry is less than 0.1 µM in buffers and cell lysates. Fluorescence microscopy demonstrated the readily detection of concentration-dependent accumulation of doxorubicin in cultured cells via either green or red fluorescence, but with green fluorescence showing a higher sensitivity of detection. Flow cytometry also revealed sensitive detection of doxorubicin accumulation in cell suspensions in a concentration-dependent manner. The readily and sensitive measurement and detection of doxorubicin by the above three fluorescence-based techniques has important implications in studying the cellular dynamics of doxorubicin in both cancer and normal cells under various experimental conditions.

Published
2016-11-01
How to Cite
Kauffman, M. K., Kauffman, M. E., Zhu, H., Jia, Z., & Li, Y. R. (2016). Fluorescence-Based Assays for Measuring Doxorubicin in Biological Systems. Reactive Oxygen Species, 2(6), 432‒439. Retrieved from https://www.aimsci.com/ros/index.php/ros/article/view/65
Section
Original Research Articles