Nrf2 Deficiency Promotes Melanoma Growth and Lung Metastasis

  • Hong Zhu Campbell University Jerry M. Wallace School of Osteopathic Medicine, Buies Creek, NC 27506, USA
  • Zhenquan Jia Department of Biology, University of North Carolina, Greensboro, NC 27412, USA
  • Michael A. Trush Department of Environmental Health Sciences, The Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD 21205, USA
  • Y. Robert Li Campbell University Jerry M. Wallace School of Osteopathic Medicine, Buies Creek, NC 27506, USA
Keywords: B16-F10 cells, Bioluminescence imaging, Bioluminometry, Lung metastasis, Melanoma, Nrf2

Abstract

The role of Nrf2, a key regulator of antioxidant and cytoprotective genes, in tumorigenesis remains controversial. Here we showed that Nrf2 deficiency led to increased local tumor growth in mice following subcutaneous injection of B16-F10 melanoma cells, as indicated by increased proportion of animals with locally palpable tumor mass and time-dependent increases in tumor volume at the injection site. In vivo bioluminescence imaging also revealed increased growth of melanoma in Nrf2-null mice as compared with wild-type mice. By using a highly sensitive bioluminometric assay, we further found that Nrf2 deficiency resulted in a remarkable increase in lung metastasis of B16-F10 melanoma cells as compared with wild-type mice. Taken together, the results of this short communication for the first time demonstrated that Nrf2 deficiency promoted melanoma growth and lung metastasis following subcutaneous inoculation of B16-F10 cells in mice.
Published
2016-05-30
How to Cite
Zhu, H., Jia, Z., Trush, M. A., & Li, Y. R. (2016). Nrf2 Deficiency Promotes Melanoma Growth and Lung Metastasis. Reactive Oxygen Species, 2(4), 308-314. Retrieved from https://www.aimsci.com/ros/index.php/ros/article/view/53
Section
Original Research Articles