Fullerene C60 Nanoparticles Decrease Liver Oxidative Stress through Increment of Liver Antioxidant Capacity in Streptozotocin-Induced Diabetes in Rats

  • Fariba Namadr Department of Physiology and Medical Physics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Farideh Bahrami Department of Physiology and Medical Physics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Zahra Bahari Department of Physiology and Medical Physics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Bahram Ghanbari Department of Chemistry, Sharif University of Technology, Tehran, Iran
  • Shima Shahyad Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  • Mohammad Taghi Mohammadi Department of Physiology and Medical Physics, School of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran; Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Keywords: Antioxidant capacity; Catalase; Diabetes; Fullerene C60; Hepatic injury; Glutathione; Lipid peroxidation; Malondialdehyde; Oxidative stress; Superoxide dismutase

Abstract

Chronic hyperglycemia causes oxidative stress in the liver and enhances the hepatic vulnerability to oxidative damage in diabetes mellitus. Since an excellent antioxidative stress property of fullerene C60 nanoparticles has been demonstrated in a wide range of in vitro and in vivo studies, we examined the effect of fullerene C60 nanoparticles on the oxidative stress markers in the liver of streptozotocin-induced diabetes in rats. Male Wistar rats were randomly divided into 4 groups (n = 8 for each group): normal, treated normal, diabetic, and treated diabetic groups. The rats were made diabetic by a single intravenous injection of streptozotocin (50 mg/kg). Treated (normal and diabetic) rats received orally fullerene C60 nanoparticles (1 mg/kg/day) by a gavage tube for 8 weeks. At termination of the study, the oxidative stress parameters were determined in liver tissues, including malondialdehyde (MDA) levels and the activities of catalase (CAT) and superoxide dismutase (SOD) as well as the content of the reduced form of glutathione (GSH). Treatment with fullerene C60 did not change blood glucose of normal and diabetic rats. Diabetes increased MDA levels and CAT activity but decreased GSH content in the liver. Fullerene C60 administration significantly decreased MDA levels and increased the activities of CAT and SOD as well as ameliorated the histopathological changes in the liver of diabetic rats. Taken together, the results of the present study indicated that fullerene C60 nanoparticles could decrease oxidative stress injury in the liver of diabetic rats likely through potentiation of the hepatic antioxidant capacity.

Published
2020-03-01
How to Cite
Namadr, F., Bahrami, F., Bahari, Z., Ghanbari, B., Shahyad, S., & Mohammadi, M. T. (2020). Fullerene C60 Nanoparticles Decrease Liver Oxidative Stress through Increment of Liver Antioxidant Capacity in Streptozotocin-Induced Diabetes in Rats. Reactive Oxygen Species, 9(26), 70–80. Retrieved from https://www.aimsci.com/ros/index.php/ros/article/view/255
Section
Original Research Articles