Superoxide in Biology and Medicine: An Overview
Robert Z. Hopkins
AIMSCI Research Institute, P.O. Box 37504, Raleigh, NC 27626, USA
Correspondence: firstname.lastname@example.org (R.Z.H.)
Hopkins RZ. Reactive Oxygen Species 1(2):99–109, 2016; ©2016 Cell Med Press
(Received: January 12, 2016; Revised: January 31, 2016; Accepted: February 1, 2016)
ABSTRACT | Since 1933 when Linus Pauling, a twice-honored Nobel laureate, proposed its existence based on the theory of quantum mechanics, superoxide has gradually taken central stage in the research field of reactive oxygen species (ROS) in biology and medicine. Indeed, superoxide is considered the primary ROS that gives rise to secondary ROS. This oxygen free radical is generated in a wide variety of biological systems ranging from aerobic microorganisms to human cells, and also formed in the deep ocean and the soils of Earth and possibly the soils of Mars as well. Superoxide is now recognized as an important molecule that is formed via defined mechanisms and involved in diverse physiological and pathophysiological processes. This article provides an overview on the basic chemistry and biochemistry of this ubiquitous oxygen free radical and its significance in biology and medicine.
KEYWORDS | Cytochrome P450 system; Fenton reaction; Haber-Weiss reaction; Mitochondria; NADPH oxidase; Reactive oxygen species; Redox cycling; Redox signaling; Superoxide; Superoxide dismutase; Uncoupled endothelial nitric oxide synthase; Xanthine oxidoreductase
ABBREVIATIONS | Cu,ZnSOD, Cu,Zn superoxide dismutase; CYP, cytochrome P450; ECSOD, extracellular superoxide dismutase; EPR, electron paramagnetic resonance; METC, mitochondrial electron transport chain; MnSOD, manganese superoxide dismutase; NOX, NAD(P)H oxidase; ROS, reactive oxygen species; SOD, superoxide dismutase; XO, xanthine oxidase
2. Sources of Superoxide
3. Chemistry and Biochemistry of Superoxide
3.1. Oxidation of Iron-Sulfur Clusters
3.2. Reaction with Nitric Oxide
3.3. Haber-Weiss Reaction and Fenton Reaction
3.4. Membrane Permeability and Protonation
4. Superoxide in Biology and Medicine
4.1. Phagocyte-Mediated Immunity
4.2. Redox Modulation and Signaling
4.3. Autoregulation of Superoxide Production
4.4. Cell and Tissue Defenses
Superoxide, also known as superoxide anion or superoxide anion radical, is designated as O2˙ˉ, where the dot denotes the unpaired electron and the minus sign denotes that the species is negatively charged. The above three names for O2˙ˉ are used interchangeably in the literature, and for simplicity, superoxide is used throughout this article. Superoxide can exist in a protonated form, known as perhydroxyl or hydroperoxyl radical (HO2˙) with a pKa of 4.8. Because of this pKa, superoxide exists predominantly in the non-protonated form (O2˙ˉ) under physiological pH.
Linus Pauling, a twice-honored Nobel laureate (Chemistry in 1954; Peace in 1962) proposed the existence of superoxide based on the theory of quantum mechanics in 1933 (see in ref. ). Two decades after Linus Pauling’s proposal, in 1954, Rebecca Gershman and associates suggested that superoxide might be responsible for both oxygen toxicity and the deleterious effects of x-irradiation . Subsequently, xanthine oxidase was proposed to produce superoxide in 1962 , and this was proved by electron paramagnetic resonance (EPR) studies in 1969 . Meanwhile, methods involving electrolytic univalent reduction of molecular oxygen were used to produce superoxide. With superoxide generated via the above approaches, Joe McCord and Irvine Fridovich discovered Cu,Zn superoxide dismutase (Cu,ZnSOD) in 1969 . Subsequently, Bernard Babior and coworkers reported superoxide generation by respiratory burst (activation of NADPH oxidase) of leukocytes as a potential mechanism of bactericidal activity . Now superoxide is considered one of the most important reactive oxygen species (ROS) that plays a significant role in a variety of biological processes and disease conditions.
2. SOURCES OF SUPEROXIDE
Superoxide is produced from one electron reduction of molecular dioxygen (oxygen for simplicity): O2 + e– → O2˙ˉ  (Figure 1). In biological systems, superoxide is generated from various metabolic processes. These include the mitochondrial electron transport chain (METC) complexes, NADPH oxidases (also known as NOXs), xanthine oxidoreductase, uncoupling of endothelial nitric oxide synthase (eNOS), and cytochrome P450 enzyme system, among others (e.g., Rac,) (Figure 2).
FIGURE 1. Electron configuration illustration of one electron reduction of molecular oxygen to superoxide. Molecular oxygen (O2) is in fact a di-radical because it contains two unpaired electrons. The same spin direction of the two unpaired electrons in O2 causes spin restriction, making O2 less reactive. On the other hand, superoxide (O2˙ˉ) contains one unpaired electron and has one more electron than O2, and as such, superoxide is negatively charged. Superoxide can become protonated to form perhydroxyl radical (HO2˙.), which is believed to be more reactive than O2˙ˉ.
FIGURE 2. Major sources of cellular superoxide. NADPH oxidases (NOXs) and mitochondria are considered the major cellular sources of superoxide. Many other enzymes and cellular processes also contribute to cellular superoxide production, such as xanthine oxidoreductase, cytochrome P450 (CYP) enzyme system (especially CYP2E1), and uncoupled endothelial nitric oxide synthase (eNOS).
Both NOXs and mitochondria are key machineries in cellular superoxide generation. Mitochondria are generally considered the chief cellular source of superoxide, whose formation is primarily derived from the univalent reduction of molecular oxygen by electrons leaked from the METC . This is not surprising as mitochondrial respiration accounts for ~90% of total cellular oxygen consumption. Recently, NOX4 is also found to be present in mitochondria and acts as a potential source of mitochondria-derived superoxide [9, 10]. Hence, mitochondria-derived superoxide is not necessarily always due to electron leakage from the METC. In addition to its presence in mitochondria, NOX4 is also found to undergo translocation into the nucleus via a leukotriene C4-dependent mechanism, and its presence in the nucleus contributes to oxidative damage to the nuclear DNA under stress conditions, including inflammatory responses and treatment with cancer chemotherapeutic agents 
Notably, xanthine oxidoreductase has two interconvertible forms, namely, xanthine dehydrogenase and xanthine oxidase (XO). Both forms catalyze the conversion of hypoxanthine to xanthine, and xanthine to uric acid. Both forms also catalyze one- and two-electron reduction of molecular oxygen to form superoxide and hydrogen peroxide (H2O2), respectively, with XO being more active in generating the above ROS. The reversible conversion of xanthine dehydrogenase to XO occurs under certain conditions including hypoxia/tissue ischemia and oxidation of the cysteine thiol groups of the enzyme. Xanthine oxidoreductase-derived ROS have been implicated in a number of pathophysiological processes, such as atherosclerosis, inflammation, and tissue ischemia-reperfusion injury in the heart, liver, and kidneys .
Cytochrome P450 (CYP) enzyme system, especially CYP2E1, is also a significant source of superoxide formation in cells, particularly under conditions when these enzymes are induced by chemicals (e.g., ethanol). CYP2E1-derived superoxide/ROS upon ethanol consumption may also be responsible for the upregulation of CYP2A5/2A6 by alcohol . Recently, a redox signaling heme-containing globin, namely, GLB-12, in Caenorhabditis elegans has been shown to generate superoxide, and the superoxide produced may play a critical signaling role in regulating apoptosis and controlling the reproduction of the organism .
In addition to the aforementioned endogenous sources, superoxide is also formed during biotransformation of certain xenobiotics, especially quinone compounds, via a process known as redox cycling (Figure 3). Chemical redox cycling may be defined as the reduction and oxidation cycle between two forms of a compound, frequently a quinone molecule. Redox cycling is typically initiated by a univalent reduction of a quinone molecule to form a semiquinone radical species. The CYP enzyme system and METC are among the major machineries that carry out the one electron reduction. The semiquinone radical formed can then donate one electron to molecular oxygen, and during this reaction, molecular oxygen is reduced to a superoxide anion radical, and the semiquinone radical is oxidized back to the original quinone molecule. Hence, the entire process involving one electron reduction and oxidation forms a cycle, leading to the persistent production of superoxide and secondary ROS (e.g., hydrogen peroxide and hydroxyl radicals).
In the sea, superoxide is generated by photolysis of organic matter in the photic zone as well as bacteria in the deep ocean with the latter being a vast source of superoxide [15, 16]. Superoxide is formed in desert soils via photochemical reactions . Superoxide may also exist in the soils of Mars. The Viking Landers were unable to detect evidence of life on Mars but, instead, found a chemically reactive soil capable of decomposing organic molecules. Using EPR spectrometry, Albert S. Yen and coworkers show that superoxide forms directly on Mars-analog mineral surfaces exposed to ultraviolet radiation under a simulated Martian atmosphere. The superoxide can explain the reactive nature of the soil and the apparent absence of organic material at the Martian surface .
FIGURE 3. Schematic illustration of the concept of chemical redox cycling. Redox cycling of quinone compounds gives rise to an enormous amount of superoxide via one electron reduction of molecular oxygen by the semiquinone radical intermediate.
3. CHEMISTRY AND BIOCHEMISTRY OF SUPEROXIDE
Although it is called “super-oxide”, superoxide is in fact not a strong oxidizing species. Because of its lower reduction potential (–330 mV for the O2/O2˙ˉ redox couple), superoxide often acts as a reducing agent rather than an oxidizing species. Indeed, the ferricytochrome c reduction assay, a method for detecting superoxide, is based on the ability of superoxide to reduce this small heme-containing protein . The ability of superoxide to directly oxidize biomolecules, including lipids, proteins, and nucleic acids is much limited. However, superoxide is an important ROS that can result in cell and tissue injury . As outlined below, the biologically damaging potential of superoxide is attributed to its several unique chemical and biochemical properties (Figure 4).
FIGURE 4. Chemical reactivity of superoxide. As illustrated, superoxide reacts with iron-sulfur clusters in enzymes, leading to enzyme inactivation. It reacts with nitric oxide (NO˙) at a diffuse-limited rate to form peroxynitrite (ONOOˉ), a potent oxidant. Superoxide is also a reactant of the iron-catalyzed Haber-Weiss reaction, giving rise to hydroxyl radical, the most potent ROS formed in biological systems. Spontaneous or superoxide dismutase (SOD)-catalyzed dismutation of superoxide produces hydrogen peroxide, another important ROS.
3.1. Oxidation of Iron-Sulfur Clusters
Although it generally acts as a reducing agent, superoxide does oxidize the iron-sulfur clusters in several enzymes, including aconitase, an enzyme of the tricarboxylic acid cycle [21, 22], and the mitochondrial electron transport chain enzyme complexes . Oxidation of the iron-sulfur clusters by superoxide in these enzymes leads to the release of iron from the enzymes and the enzyme inactivation. The released iron ions may participate in the Fenton reaction, resulting in the formation of hydroxyl radicals (see Section 3.3 below). In contrast, reversible oxidation of the iron-sulfur cluster in prokaryotic transcription factors SoxR/SoxS leads to their activation (see Section 4.2 below).
3.2. Reaction with Nitric Oxide
Superoxide reacts with nitric oxide (NO˙) at an almost diffusion-limited rate to form peroxynitrite anion (ONOOˉ), which is frequently called peroxynitrite for simplicity. The reaction is as following: O2˙ˉ + NO˙ → ONOOˉ. Peroxynitrite is a potent oxidant that causes damage to various biomolecules . The fast reaction between superoxide and nitric oxide also contributes to the decreased bioavailability of nitric oxide under diverse pathophysiological conditions. Nitric oxide is generally regarded as a cardiovascular protective molecule, whose deficiency might contribute, at least partly, to such diseases as atherosclerosis, hypertension, coronary artery disease, and erectile dysfunction 
3.3. Haber-Weiss Reaction and Fenton Reaction
In the presence of transition metal ions, such as iron (Fe) ions, superoxide and hydrogen peroxide together can give rise to hydroxyl radical (OH˙), the most potent ROS capable of damaging the entire spectrum of biomolecules. The reaction of superoxide and hydrogen peroxide in the presence of Fe ions to produce hydroxyl radical is known as iron-catalyzed Haber-Weiss reaction, which was first proposed by Fritz Haber and Joseph Weiss in 1934  (Figure 5). In addition to his contributions to the field of free radical biology, Fritz Haber received the Nobel Prize in Chemistry in 1918 for the synthesis of ammonia from its elements.
In the absence of Fe ions, the above reaction proceeds slowly. The presence of Fe ions markedly accelerates the reaction to produce hydroxyl radical. The Fe ion-catalyzed Haber-Weiss reaction can be written in two sequential sub-reactions: (1) O2˙ˉ + Fe3+ → O2 + Fe2+, and (2) Fe2+ + H2O2 → Fe3+ + OH˙ + OHˉ. The second reaction is commonly referred to as the Fenton reaction, which is also frequently called the Fenton chemistry.
By definition, Fenton chemistry refers to the oxidation of organic substrates by Fe2+ and H2O2. In 1894, Henry J.H. Fenton first observed the oxidation of tartaric acid by H2O2 in the presence of Fe2+ , indicating that a potent oxidant(s) is formed by the reaction between H2O2 and Fe2+, which are also known as the Fenton reagent. The Fenton reagent is commonly used to oxidize (destroy) organic chemicals, such as wastes in water. Later in the 1930s, Fritz Haber and Joseph Weiss proposed the formation of hydroxyl radical from the Fenton reagent, which is known today to be a major ultimate species responsible for oxidative damage of a wide range of biomolecules.
In addition to Fe2+, other transition metal ions, such as cuprous ion (Cu1+), also react with H2O2, forming hydroxyl radical or its equivalent: M(n-1)+ + H2O2 → Mn+ + OH˙ + OHˉ, where M denotes the transition metal ion. For example, Cu1+ + H2O2 → Cu2+ + OH˙ + OHˉ. Such reactions are commonly referred to as Fenton-type reactions.
FIGURE 5. Iron-catalyzed Haber-Weiss reaction. The reaction between superoxide and H2O2 to form hydroxyl radical is commonly referred to as the Haber-Weiss reaction. This reaction proceeds slowly and can be dramatically accelerated by the presence of iron (Fe) ions. The reaction on the right side of the scheme is also called the Fenton reaction.
3.4. Membrane Permeability and Protonation
Another factor influencing the biological activities of superoxide is its limited ability to cross biomembranes due to the negative charge and short half-life of this free radical species. However, superoxide may cross cell membranes through anion channels, such as the 4-diisothiocyano-2,2-disulfonic acid stilbene (DIDS)-sensitive chloride channel . In contrast, perhydroxyl radical, the protonated form of superoxide, is highly membrane permeable, and is also a potent oxidizing species . Perhydroxyl radical has a pKa of 4.8, and as such, may reach high levels in cellular compartments with high concentrations of protons, including the mitochondrial intermembrane space. A recent study by Michael A. Trush and coworkers provides direct evidence for the mitochondrial electron transport chain-derived superoxide to exit mitochondria and cross cell membranes to enter the extracellular space . Exit of superoxide from mitochondria and its subsequent crossing of cell membranes to enter extracellular milieu provide a basis for superoxide to participate in diverse intracellular and intercellular processes under physiological and pathophysiological conditions.
4. SUPEROXIDE IN BIOLOGY AND MEDICINE
Superoxide is one of the most extensively investigated ROS in biology and medicine. A PubMed search on January 30, 2016, revealed 82,289 entries containing the term ‘superoxide’ in title/abstract. The number of entries for ‘hydrogen peroxide’ and ‘hydroxyl radical’ was 42,193 and 10,008, respectively. As outlined below, superoxide anion radical plays an important role in both physiological and pathophysiological processes.
4.1. Phagocyte-Mediated Immunity
Superoxide produced from phagocytic NADPH oxidase during respiratory burst is an important precursor molecule leading to the formation of more potent ROS, especially hypochlorous acid (HOCl) and peroxynitrite that kill the invading microorganisms. Dismutation of superoxide generates hydrogen peroxide, which in turn reacts with chloride ion in the presence of the phagocyte-derived myeloperoxidase (MPO), forming hypochlorous acid. Likewise, superoxide released from the NADPH oxidase reacts with nitric oxide generated by inducible nitric oxide synthase (iNOS), resulting in the formation of peroxynitrite. Failure to generate phagocyte-derived superoxide and related ROS is the major defect in chronic granulomatous disease, causing recurrent infections and granulomatous complications .
4.2. Redox Modulation and Signaling
The role of superoxide in redox regulation in prokaryotes is well established. In bacteria, SoxR and SoxS are activated by superoxide via reversible one-electron oxidation of the [2Fe-2S] cluster and then enhance the production of various antioxidant proteins through the soxRS regulon [31, 32].
Superoxide stimulates cell proliferation. Overexpression of superoxide-producing NADPH oxidase induces cell transformation  though the responsible ROS seems to be hydrogen peroxide derived from the dismutation of superoxide . Superoxide may also relay Ras protein’s oncogenic message .
4.3. Autoregulation of Superoxide Production
Superoxide may regulate its own production. On the one hand, superoxide activates mitochondrial uncoupling proteins, leading to decreased formation of superoxide from the mitochondrial electron transport chain [36, 37]. On the other hand, superoxide flux across the endothelial cell plasma membrane occurs through chloride ion channels and induces intracellular calcium ion release, which in turn activates mitochondrial superoxide generation .
4.4. Cell and Tissue Defenses
Superoxide undergoes spontaneous dismutation to form hydrogen peroxide and molecular oxygen with a reaction rate constant of ~5 × 105 M‒1s‒1 at pH 7.0 (Figure 6) . The term dismutation reaction refers to a chemical reaction in which the same reactant is both oxidized and reduced. In superoxide dismutation reaction, one molecule of superoxide is oxidized to molecular oxygen, and another is reduced to hydrogen peroxide. The dismutation reaction of superoxide is catalyzed by superoxide dismutase (SOD) with a reaction rate constant of ~1.6 × 109 M‒1s‒1 which is over three orders of magnitude greater than that of spontaneous dismutation (Figure 6).
In mammalian species, including humans, there are three types of SOD: (1) Cu,ZnSOD, (2) manganese (MnSOD), and (3) extracellular SOD (ECSOD). All three forms of SOD catalyze dismutation of superoxide to hydrogen peroxide and molecular oxygen at a similar reaction rate constant.
Recently, NAD(P)H oxidoreductase 1 (NQO1), an enzyme that catalyzes 2-electrone reduction of quinone compounds, has been suggested to scavenge biological superoxide possibly by catalyzing its conversion to hydrogen peroxide [39, 40]. NQO1 appears to play a role in scavenging superoxide in tissues, such as vascular endothelium and myocardium, where SOD is relatively deficient and NQO1 is, on the other hand, highly expressed . The fate of cellular superoxide is illustrated in Figure 7.
FIGURE 6. Spontaneous and SOD-catalyzed dis-mutation of superoxide. As illustrated, the dismutation reaction of superoxide is greatly accelerated by SOD with a reaction rate constant being over three orders of magnitude greater than that of spontaneous dismutation.
FIGURE 7. The cellular fate of mitochondria-derived superoxide. Mitochondrial electron transport chain (METC) is the major source of cellular superoxide formation. The METC-derived superoxide undergoes MnSOD-catalyzed dismutation to form H2O2, which is then converted to water by glutathione peroxidase (GPx) or peroxiredoxin (Prx) in mitochondrial matrix. Superoxide is able to cross the mitochondrial inner membrane and enter the cytosol, where Cu,ZnSOD catalyzes its dismutation to H2O2, and the H2O2 formed in the cytosol or escaped from mitochondria is converted to water by enzymes, including GPx, Prx, and catalase (CAT), in the cytoplasm. CuZnSOD present in the mitochondrial intermembrane space also catalyzes the dismutation of superoxide present in the intermembrane space. ECSOD acts on extracellular superoxide.
Although superoxide possesses physiological functions, dysregulated formation of this oxygen radical leads to tissue injury. In fact, formation of superoxide is an important mechanism of oxygen toxicity. The tissue damage may occur as a result of direct effects of superoxide on molecular targets but more likely due to the secondary formation of hydrogen peroxide from superoxide dismutation or the formation of peroxynitrite from the reaction of superoxide with nitric oxide (Figure 4).
The causal involvement of superoxide in disease pathogenesis is supported by numerous studies showing that genetic deletion of SOD causes diverse disease processes and transgenic overexpression of SOD renders resistance to disease pathogenesis in animal models [41–43]. The diseases or conditions whose pathogenesis is impacted by the alterations of SOD expression or content involve diverse organs and systems, and include aging [44, 45], cancer [46–48], cardiovascular diseases [49, 50], diabetes [42, 51], neurodegeneration [52, 53], immunological disorders [54, 55], pulmonary disorders [56, 57], hepatic disorders [58, 59], gastrointestinal diseases [60, 61], and kidney diseases [51, 62, 63].
Although substantial evidence supports a causal involvement of superoxide in diverse disease processes in animal models, the exact role of superoxide and SOD in disease pathogenesis in humans remains to be established. The only human disease that is, thus far, known to be affected by mutations in the SOD (Cu,ZnSOD) gene is familial amyotrophic lateral sclerosis . Large-scale, well-designed, randomized controlled trials on using SOD in human disease intervention are currently lacking. Clinical research on the role of superoxide in disease process is hampered by the limited bioavailability of native SOD. In this context, recent development in selective SOD biomimetics with favorable pharmacokinetic properties  would facility research on the role of superoxide in human diseases.
The past several decades have witnessed substantial advances in understanding the chemistry, biology, and medicine of superoxide. Its ubiquitous presence in biological systems, its formation via defined cellular processes, and its unique biochemical properties make superoxide an important factor involved in diverse physiological and pathophysiological processes. Ongoing efforts on both basic and clinical studies of superoxide will further increase our understanding of this ROS with regard to its significance in biology and medicine. The coming years will likely witness the translation of the new knowledge learned from studies with experimental models into the development of mechanistically-based strategies for modulating superoxide-mediated biological effects in humans so as to minimize its deleterious effects while maintaining the physiological function of this oxygen free radical.
- Pauling L. The discovery of the superoxide radical. Trends Biochem Sci 1979; 4(11):N270–N1. doi: 10.1016/0968-0004(79)90203-2.
- Gerschman R, Gilbert DL, Nye SW, Dwyer P, Fenn WO. Oxygen poisoning and x-irradiation: a mechanism in common. Science 1954; 119(3097):623–6.
- Fridovich I, Handler P. Xanthine oxidase. V. Differential inhibition of the reduction of various electron acceptors. J Biol Chem 1962; 237:916–21.
- Knowles PF, Gibson JF, Pick FM, Bray RC. Electron-spin-resonance evidence for enzymic reduction of oxygen to a free radical, the superoxide ion. Biochem J 1969; 111(1):53–8.
- McCord JM, Fridovich I. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244(22):6049–55.
- Babior BM, Kipnes RS, Curnutte JT. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest 1973; 52(3):741–4. doi: 10.1172/JCI107236.
- Li YR, Jia Z, Trush MA. Defining ROS in biology and medicine. Reactive Oxygen Species 2016; 1(1):9–21. doi: 10.20455/ros.2016.803.
- Li Y, Zhu H, Kuppusamy P, Zweier JL, Trush MA. Mitochondrial electron transport chain-derived superoxide exits macrophages: implications for mononuclear cell-mediated pathophysiological processes. Reactive Oxygen Species 2016; 1(1):81–98. doi: 10.20455/ros.2016.815.
- Block K, Gorin Y, Abboud HE. Subcellular localization of Nox4 and regulation in diabetes. Proc Natl Acad Sci USA 2009; 106(34):14385–90. doi: 10.1073/pnas.0906805106.
- Kuroda J, Ago T, Matsushima S, Zhai P, Schneider MD, Sadoshima J. NADPH oxidase 4 (Nox4) is a major source of oxidative stress in the failing heart. Proc Natl Acad Sci USA 2010; 107(35):15565–70. doi: 10.1073/pnas.1002178107.
- Dvash E, Har-Tal M, Barak S, Meir O, Rubinstein M. Leukotriene C4 is the major trigger of stress-induced oxidative DNA damage. Nat Commun 2015; 6:10112. doi: 10.1038/ncomms10112.
- McCord JM. Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med 1985; 312(3):159–63. doi: 10.1056/NEJM198501173120305.
- Lu Y, Cederbaum AI. Alcohol upregulation of CYP2A5: role of reactive oxygen species. Reactive Oxygen Species 2016; 1(2):117-30. doi: 10.20455/ros.2016.823.
- De Henau S, Tilleman L, Vangheel M, Luyckx E, Trashin S, Pauwels M, et al. A redox signalling globin is essential for reproduction in Caenorhabditis elegans. Nat Commun 2015; 6:8782. doi: 10.1038/ncomms9782.
- Shaked Y, Rose A. Microbiology. Seas of superoxide. Science 2013; 340(6137):1176–7. doi: 10.1126/science.1240195.
- Diaz JM, Hansel CM, Voelker BM, Mendes CM, Andeer PF, Zhang T. Widespread production of extracellular superoxide by heterotrophic bacteria. Science 2013; 340(6137):1223–6. doi: 10.1126/science.1237331.
- Georgiou CD, Sun HJ, McKay CP, Grintzalis K, Papapostolou I, Zisimopoulos D, et al. Evidence for photochemical production of reactive oxygen species in desert soils. Nat Commun 2015; 6:7100. doi: 10.1038/ncomms8100.
- Yen AS, Kim SS, Hecht MH, Frant MS, Murray B. Evidence that the reactivity of the martian soil is due to superoxide ions. Science 2000; 289(5486):1909–12.
- Zhu H. Assays for detecting biological superoxide. Reactive Oxygen Species 2016; 1(1):65–80. doi: 10.20455/ros.2015.813.
- Fridovich I. Superoxide anion radical (O2-.), superoxide dismutases, and related matters. J Biol Chem 1997; 272(30):18515–7.
- Gardner PR, Fridovich I. Superoxide sensitivity of the Escherichia coli aconitase. J Biol Chem 1991; 266(29):19328–33.
- Gardner PR, Nguyen DD, White CW. Aconitase is a sensitive and critical target of oxygen poisoning in cultured mammalian cells and in rat lungs. Proc Natl Acad Sci USA 1994; 91(25):12248–52.
- Diaz F, Enriquez JA, Moraes CT. Cells lacking Rieske iron-sulfur protein have a reactive oxygen species-associated decrease in respiratory complexes I and IV. Mol Cell Biol 2012; 32(2):415–29. doi: 10.1128/MCB.06051-11.
- Radi R. Peroxynitrite, a stealthy biological oxidant. J Biol Chem 2013; 288(37):26464–72. doi: 10.1074/jbc.R113.472936.
- Murad F. Shattuck Lecture. Nitric oxide and cyclic GMP in cell signaling and drug development. N Engl J Med 2006; 355(19):2003–11. doi: 10.1056/NEJMsa063904.
- Haber F, Weiss J. The catalytic decomposition of hydrogen peroxide by iron salts. Proc R Soc A 1934; 147(861):332–51. doi: 10.1098/rspa.1934.0221
- Fenton HJ. Oxidation of tartaric acid in presence of iron. J Chem Soc, Trans 1894; 65, 899-910. doi: 10.1039/CT8946500899.
- Mumbengegwi DR, Li Q, Li C, Bear CE, Engelhardt JF. Evidence for a superoxide permeability pathway in endosomal membranes. Mol Cell Biol 2008; 28(11):3700–12. doi: 10.1128/MCB.02038-07.
- De Grey AD. HO2˙: the forgotten radical. DNA Cell Biol 2002; 21(4):251–7. doi: 10.1089/104454902753759672.
- Kuhns DB, Alvord WG, Heller T, Feld JJ, Pike KM, Marciano BE, et al. Residual NADPH oxidase and survival in chronic granulomatous disease. N Engl J Med 2010; 363(27):2600–10. doi: 10.1056/NEJMoa1007097.
- Watanabe S, Kita A, Kobayashi K, Miki K. Crystal structure of the [2Fe-2S] oxidative-stress sensor SoxR bound to DNA. Proc Natl Acad Sci USA 2008; 105(11):4121–6. doi: 10.1073/pnas.0709188105.
- Demple B, Amabile-Cuevas CF. Redox redux: the control of oxidative stress responses. Cell 1991; 67(5):837–9.
- Suh YA, Arnold RS, Lassegue B, Shi J, Xu X, Sorescu D, et al. Cell transformation by the superoxide-generating oxidase Mox1. Nature 1999; 401(6748):79–82. doi: 10.1038/43459.
- Arnold RS, Shi J, Murad E, Whalen AM, Sun CQ, Polavarapu R, et al. Hydrogen peroxide mediates the cell growth and transformation caused by the mitogenic oxidase Nox1. Proc Natl Acad Sci USA 2001; 98(10):5550–5. doi: 10.1073/pnas.101505898.
- Pennisi E. Superoxides relay Ras protein’s oncogenic message. Science 1997; 275(5306):1567–8.
- Echtay KS, Roussel D, St-Pierre J, Jekabsons MB, Cadenas S, Stuart JA, et al. Superoxide activates mitochondrial uncoupling proteins. Nature 2002; 415(6867):96–9. doi: 10.1038/415096a.
- Echtay KS, Murphy MP, Smith RA, Talbot DA, Brand MD. Superoxide activates mitochondrial uncoupling protein 2 from the matrix side: studies using targeted antioxidants. J Biol Chem 2002; 277(49):47129–35. doi: 10.1074/jbc.M208262200.
- Hawkins BJ, Madesh M, Kirkpatrick CJ, Fisher AB. Superoxide flux in endothelial cells via the chloride channel-3 mediates intracellular signaling. Mol Biol Cell 2007; 18(6):2002–12. doi: 10.1091/mbc.E06-09-0830.
- Siegel D, Gustafson DL, Dehn DL, Han JY, Boonchoong P, Berliner LJ, et al. NAD(P)H:quinone oxidoreductase 1: role as a superoxide scavenger. Mol Pharmacol 2004; 65(5):1238–47. doi: 10.1124/mol.65.5.1238.
- Zhu H, Jia Z, Mahaney JE, Ross D, Misra HP, Trush MA, et al. The highly expressed and inducible endogenous NAD(P)H:quinone oxidoreductase 1 in cardiovascular cells acts as a potential superoxide scavenger. Cardiovasc Toxicol 2007; 7(3):202–11. doi: 10.1007/s12012-007-9001-z.
- Melov S, Schneider JA, Day BJ, Hinerfeld D, Coskun P, Mirra SS, et al. A novel neurological phenotype in mice lacking mitochondrial manganese superoxide dismutase. Nat Genet 1998; 18(2):159–63. doi: 10.1038/ng0298-159.
- Shen X, Zheng S, Metreveli NS, Epstein PN. Protection of cardiac mitochondria by overexpression of MnSOD reduces diabetic cardiomyopathy. Diabetes 2006; 55(3):798–805.
- Li Y, Huang TT, Carlson EJ, Melov S, Ursell PC, Olson JL, et al. Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase. Nat Genet 1995; 11(4):376–81. doi: 10.1038/ng1295-376.
- Orr WC, Sohal RS. Extension of life-span by overexpression of superoxide dismutase and catalase in Drosophila melanogaster. Science 1994; 263(5150):1128–30.
- Melov S, Ravenscroft J, Malik S, Gill MS, Walker DW, Clayton PE, et al. Extension of life-span with superoxide dismutase/catalase mimetics. Science 2000; 289(5484):1567–9.
- Kim SH, Kim MO, Gao P, Youm CA, Park HR, Lee TS, et al. Overexpression of extracellular superoxide dismutase (EC-SOD) in mouse skin plays a protective role in DMBA/TPA-induced tumor formation. Oncol Res 2005; 15(7–8):333–41.
- Kensler TW, Bush DM, Kozumbo WJ. Inhibition of tumor promotion by a biomimetic superoxide dismutase. Science 1983; 221(4605):75–7.
- Van Remmen H, Ikeno Y, Hamilton M, Pahlavani M, Wolf N, Thorpe SR, et al. Life-long reduction in MnSOD activity results in increased DNA damage and higher incidence of cancer but does not accelerate aging. Physiol Genomics 2003; 16(1):29–37. doi: 10.1152/physiolgenomics.00122.2003.
- Yen HC, Oberley TD, Vichitbandha S, Ho YS, St Clair DK. The protective role of manganese superoxide dismutase against adriamycin-induced acute cardiac toxicity in transgenic mice. J Clin Invest 1996; 98(5):1253–60. doi: 10.1172/JCI118909.
- Lebovitz RM, Zhang H, Vogel H, Cartwright J, Jr., Dionne L, Lu N, et al. Neurodegeneration, myocardial injury, and perinatal death in mitochondrial superoxide dismutase-deficient mice. Proc Natl Acad Sci USA 1996; 93(18):9782–7.
- Kubisch HM, Wang J, Luche R, Carlson E, Bray TM, Epstein CJ, et al. Transgenic copper/zinc superoxide dismutase modulates susceptibility to type I diabetes. Proc Natl Acad Sci USA 1994; 91(21):9956–9.
- Murakami K, Murata N, Noda Y, Tahara S, Kaneko T, Kinoshita N, et al. SOD1 (copper/zinc superoxide dismutase) deficiency drives amyloid beta protein oligomerization and memory loss in mouse model of Alzheimer disease. J Biol Chem 2011; 286(52):44557–68. doi: 10.1074/jbc.M111.279208.
- Massaad CA, Washington TM, Pautler RG, Klann E. Overexpression of SOD-2 reduces hippocampal superoxide and prevents memory deficits in a mouse model of Alzheimer’s disease. Proc Natl Acad Sci USA 2009; 106(32):13576–81. doi: 10.1073/pnas.0902714106.
- Marikovsky M, Ziv V, Nevo N, Harris-Cerruti C, Mahler O. Cu/Zn superoxide dismutase plays important role in immune response. J Immunol 2003; 170(6):2993–3001.
- Iuchi Y, Okada F, Takamiya R, Kibe N, Tsunoda S, Nakajima O, et al. Rescue of anaemia and autoimmune responses in SOD1-deficient mice by transgenic expression of human SOD1 in erythrocytes. Biochem J 2009; 422(2):313–20. doi: 10.1042/BJ20090176.
- Folz RJ, Abushamaa AM, Suliman HB. Extracellular superoxide dismutase in the airways of transgenic mice reduces inflammation and attenuates lung toxicity following hyperoxia. J Clin Invest 1999; 103(7):1055–66. doi: 10.1172/JCI3816.
- Yao H, Arunachalam G, Hwang JW, Chung S, Sundar IK, Kinnula VL, et al. Extracellular superoxide dismutase protects against pulmonary emphysema by attenuating oxidative fragmentation of ECM. Proc Natl Acad Sci USA 2010; 107(35):15571–6. doi: 10.1073/pnas.1007625107.
- Wheeler MD, Nakagami M, Bradford BU, Uesugi T, Mason RP, Connor HD, et al. Overexpression of manganese superoxide dismutase prevents alcohol-induced liver injury in the rat. J Biol Chem 2001; 276(39):36664–72. doi: 10.1074/jbc.M105352200.
- Laukkanen MO, Leppanen P, Turunen P, Tuomisto T, Naarala J, Yla-Herttuala S. EC-SOD gene therapy reduces paracetamol-induced liver damage in mice. J Gene Med 2001; 3(4):321–5. doi: 10.1002/jgm.194.
- Carroll IM, Andrus JM, Bruno-Barcena JM, Klaenhammer TR, Hassan HM, Threadgill DS. Anti-inflammatory properties of Lactobacillus gasseri expressing manganese superoxide dismutase using the interleukin 10-deficient mouse model of colitis. Am J Physiol Gastrointest Liver Physiol 2007; 293(4):G729–38. doi: 10.1152/ajpgi.00132.2007.
- Horie Y, Wolf R, Flores SC, McCord JM, Epstein CJ, Granger DN. Transgenic mice with increased copper/zinc-superoxide dismutase activity are resistant to hepatic leukostasis and capillary no-reflow after gut ischemia/reperfusion. Circ Res 1998; 83(7):691–6.
- DeRubertis FR, Craven PA, Melhem MF, Salah EM. Attenuation of renal injury in db/db mice overexpressing superoxide dismutase: evidence for reduced superoxide− nitric oxide interaction. Diabetes 2004; 53(3):762–8.
- Yin M, Wheeler MD, Connor HD, Zhong Z, Bunzendahl H, Dikalova A, et al. Cu/Zn-superoxide dismutase gene attenuates ischemia-reperfusion injury in the rat kidney. J Am Soc Nephrol 2001; 12(12):2691–700.
- Rosen DR, Siddique T, Patterson D, Figlewicz DA, Sapp P, Hentati A, et al. Mutations in Cu/Zn superoxide dismutase gene are associated with familial amyotrophic lateral sclerosis. Nature 1993; 362(6415):59–62. doi: 10.1038/362059a0.
- Bird HZ, Hopkins RZ. Nanomaterials for selective superoxide dismutation. Reactive Oxygen Species 2016; 1(1):59–64. doi: 10.20455/ros.2016.811.